Role of Transportin-1 in the uncoating and nuclear entry of viruses

Web seminar of the 3I (Infection, Immunity and Inflammation) department
  • Quand ? 22/06/2020 de 12:00 à 13:00 (Europe/Berlin / UTC200)
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Nathalie Arhel


Equipe VTRIS (Viral Trafficking, Restriction and Innate Signaling), Unité IRIM (Institut de Recherche en Infectiologie de Montpellier), CNRS UMR9004/Université de Montpellier.

 

Summary: HIV-1 uncoating is defined as the loss of viral capsid that occurs following virus entry into a cell and prior to integration into the genome. Many papers have demonstrated that the virus must disassemble at the right time and in the right place for the nuclear import of the viral genome to succeed. However, the mechanisms involved remain unclear. In a recent study (Nat Microbiol, Nov 2019, 4:1840-1850), we showed that the nucleocytoplasmic soluble transport protein called Transportin (TRN-1, gene name TNPO1) is an important cellular co-factor of HIV-1 infection: it binds to incoming capsids, triggers their uncoating and promotes viral nuclear import. Knock-down of TRN-1 significantly reduces HIV-1 infection, and dependency on TRN-1 maps to the cyclophilin A-binding loop on CA, which contains a bona fide nuclear localization signal (NLS). Intringingly, TRN-1 is also involved in the nuclear import of adenovirus or influenza virus...